Coding
hERG

Part:BBa_K2263001:Design

Designed by: Imke Ensinck   Group: iGEM17_KU_Leuven   (2017-10-25)


hERG - human Ether-a-go-go-Related Gene K+ channel


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1689
    Illegal NheI site found at 1957
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1913
    Illegal XhoI site found at 2089
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2762
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3157
    Illegal SapI.rc site found at 2934


Design Notes

We have adapted the coding sequence to conform to the biobrick standard. In order to achieve this standard, multiple illegal restriction sites have been removed by silent mutations. Furthermore, the GC content of certain regions of the gene was lowered by silent mutations, in order to facilitate synthesis in gBlock parts.


Source

It was adapated from human mRNA isoform 1 sequence of the kcnh2 gene.

References

Further reading on hERG: Michael C. Sanguinetti, Martin Tristani-Firouzi. hERG potassium channels and cardiac arrhythmia. (2006) Nature 440, 463-469